SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active), Unconjugated

Catalog Number: GTX136059-PRO
Article Name: SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active), Unconjugated
Biozol Catalog Number: GTX136059-PRO
Supplier Catalog Number: GTX136059-pro
Alternative Catalog Number: GTX136059-PRO-100
Manufacturer: GeneTex
Category: Proteine/Peptide
Application: ELISA, Receptor Binding Assays, WB
Species Reactivity: Virus
Conjugation: Unconjugated
Alternative Names: Alpha variant Spike, B.1.1.7 Spike, COVID-19 Alpha variant Spike, SARS-CoV-2 Alpha variant Spike, covid-19, sars-cov-2
SARS-CoV-2 (COVID-19) Spike (ECD) Protein, B.1.1.7 / Alpha variant, His tag (active), Unconjugated
Concentration: 0.33 mg/ml (Please refer to the vial label for the specific concentration.)
Buffer: PBS, no Preservative.
Expression System: HEK293 cells
Form: Liquid
Sequence: SARS-CoV-2 Spike of QHD43416.1 (1-1213 a.a, extracellular domain) with UK variant (501Y.V1 / B.1.1.7) mutations (deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H ), S1-S2 cleavage site mutations (682-685GSAS), 6 stabilizing
Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (active) (UK variant) (GTX136059-pro), SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike Protein (Wild type) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag (GTX136059-pro, 0.25 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
1 μg of GTX136059-pro SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...,D1118H) (ECD) Protein, His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.
GTX136059-pro ELISA Image
Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 μg/mL)
Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 μg/mL)
Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) (1 μg/mL)
Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 μg/mL)
Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 μg/mL)
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.