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WB analysis of untreated or Nocodazole (100nM, 48 hours) treated U2OS lysate from non-targeting control or PLK1 siRNA transfected U2OS cells using GTX15779 PLK1 antibody [13E8]. Dilution : 1:1000 Loading : 25 μg |
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IHC-P analysis of human colon tissue using GTX15779 PLK1 antibody [13E8]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 |
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ICC/IF analysis of U251 cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC |
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GTX15779 WB Image |
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ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC |
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WB analysis of PLK1 knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15779 PLK1 antibody [13E8]. Dilution : 1 μg/ml |
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ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Progression of cells through subsequent phases of the cell cycle results in differential localization of PLK1 in spindle poles, kinetochores and cleavage furrow. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 3 μg/ml in 0.1% BSA and incubated for overnight at 4ºC |
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ICC/IF analysis of WiDr cells using GTX15779 PLK1 antibody [13E8]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC |
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ICC/IF analysis of HeLa cells using GTX15779 PLK1 antibody [13E8]. Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 3 μg/ml in 0.1% BSA and incubated for overnight at 4ºC |