Human alpha Synuclein protein (active, Pre-Formed Fibrils), Unconjugated

Product Overview

• Article Name: Human alpha Synuclein protein (active, Pre-Formed Fibrils), Unconjugated
• Biozol Catalog Number: GTX17669-PRO
• Supplier Catalog Number: GTX17669-pro
• Alternative Catalog Number: GTX17669-PRO-200,GTX17669-PRO-100
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Human
• Conjugation: Unconjugated
• Alternative Names: synuclein alpha , synuclein alpha , NACP , PARK1 , PARK4 , PD1

Product Description

Active Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 1)

Product Properties

• Concentration: Batch dependent (Please refer to the vial label for the specific concentration.)
• NCBI: 6622
• Buffer: PBS, no preservative.
• Expression System: E. coli
• Purity: Purified by ion-exchange chromatography
• Form: Liquid
• Sequence: Full-length without tagged, MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Application Notes

• Application Notes: Endogenous alpha-synuclein phosphorylation. 100 µM alpha synuclein protein monomer (GTX17668-pro) seeded with 10 nM alpha synuclein protein PFF (GTX17669-pro) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity o

Images

GTX17669-pro Functional Assay Image

SDS-PAGE of ~14 kDa active Human Recombinant Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro). Lane 1: Molecular Weight Ladder (MW). Lane 2: active Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro).

Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro) at 4 μg/ml (D-F), but not when treated with control Alpha Synuclein Protein Preformed Fibrils (GTX17667-pro) at 4 μg/ml (A-C).
Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4ºC. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body inclusions. Magnification: 20x.

Active alpha synuclein preformed fibrils (GTX17669-pro) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (GTX17668-pro). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of active alpha synuclein preformed fibrils (GTX17669-pro) is combined with 100 μM of alpha synuclein monomer (GTX17668-pro), as compared to active alpha synuclein preformed fibrils (GTX17669-pro) alone and alpha synuclein monomer (GTX17668-pro) alone. Thioflavin T ex = 450 nm, em = 485 nm.