easy iPSC
Catalog Number:
GTX400001
- Images (8)
| Article Name: | easy iPSC |
| Biozol Catalog Number: | GTX400001 |
| Supplier Catalog Number: | GTX400001 |
| Alternative Catalog Number: | GTX400001-100 |
| Manufacturer: | GeneTex |
| Category: | Sonstiges |
| Application: | ICC, Cell Culture, RT-PCR |
| Species Reactivity: | Human, Mouse |
| Alternative Names: | minicircle DNA , easy-iPSC , easyiPSC , iPSC Minicircle , easy iPSC |
| Minicircle DNA for iPSC Production. Easy iPSC is a simple non-viral solution for reprogramming terminally differentiated cells into pluripotent stem cells. One simple transfection delivers all four transcription factors required. This specially designed |
| Concentration: | 1 mg/ml (Please refer to the vial label for the specific concentration.) |
| Buffer: | 0.1X TE Buffer, no Preservative. |
| Form: | Liquid |
| Application Notes: | This protocol has been optimized for use in human adipose stem cells (hASC). Use in other cell types, such as fibroblasts, will require optimization by the researcher. |
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RT-PCR analysis of markers for pluripotency and each germ layer confirms that easy iPSC-transfected hASC can be induced to differentiate into cells of each germ layer. Image from Jia et al. 2010. Nature Methods, 7(3). |
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easy iPSC induces the expression of endogenous OCT4 in hASC as determined by RT-PCR. Primers specific for the 3' UTR of endogenous OCT4 (GTX80102) were used for analysis. Image from Jia et al. 2010. Nature Methods, 7(3). |
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hASC transfected with easy iPSC express pluripotency markers. Image from Jia et al. 2010. Nature Methods, 7(3). |
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