Methylated cytosine DNA standard kit

Catalog Number: GTX400004
Article Name: Methylated cytosine DNA standard kit
Biozol Catalog Number: GTX400004
Supplier Catalog Number: GTX400004
Alternative Catalog Number: GTX400004-1
Manufacturer: GeneTex
Category: Kits/Assays
Application: DOT
Alternative Names: 5-methylcytosine , 5-hydroxymethylcytosine , 5-formylcytosine , 5-carboxylcytosine
Concentration: 50 ng/µl (Please refer to the vial label for the specific concentration.)
Buffer: 10mM Tris-HCl, 0.1mM EDTA, pH 8.0. All standards are supplied in 40 µl volume.
GeneTex methylated cytosine DNA standard kit includes five recombinant double-strand DNA fragments: unmethylated cytosine DNA (C), 5-methylated cytosine DNA (5mC), 5-hydroxymethycytosine cytosine DNA (5hmC), 5-formylcytosine cytosine DNA (5fC), 5-carboxylcytosine cytosine DNA (5caC). Each methylated cytosine DNA was spotted onto a positively charged nylon membrane and subjected to Dot blot assay with corresponding antibodies.
The membrane was incubated with anti-5hmc monoclonal antibody (GTX629701) at a dilution of 1:500 or with anti-5mc monoclonal antibody (GTX629448) at a dilution of 1:500
All five standards are 426 bp with all cytosines either unmodified (lane 1, C), 5-methylcytosine (lane 2, 5mC), 5-hydroxymethylcytosine (lane 3, 5hmC), 5-formylcytosin (lane4, 5fC), 5-carboxycytosine (lane5, 5-caC). DNA marker: 1 kb Plus DNA marker.