STAT1 antibody [4H9], IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: STAT1 antibody [4H9], IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX83552
• Supplier Catalog Number: GTX83552
• Alternative Catalog Number: GTX83552-100
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Canine, Human, Monkey
• Immunogen: Full length human recombinant protein of human STAT1 (NP_009330) produced in HEK293T cell.
• Conjugation: Unconjugated
• Alternative Names: signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF-3 , STAT91

Product Description

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylatio

Product Properties

• Clonality: Monoclonal
• Concentration: 0.7 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [4H9]
• Molecular Weight: 87
• Isotype: IgG1
• NCBI: 6772
• UniProt: P42224
• Buffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
• Source: Human
• Purity: Purified by affinity chromatography
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000-1:2000. ICC/IF: 1:50-1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83552 STAT1 antibody [4H9].
Loading : 10 ug per lane
Dilution : 1:200

GTX83552 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

IHC-P analysis of human kidney tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

ICC/IF analysis of HT29 cells using GTX83552 STAT1 antibody [4H9].

IHC-P analysis of human endometrium tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

WB analysis of various cell lines using GTX83552 STAT1 antibody [4H9].
Loading : 35 ug per lane

WB analysis of HEK293T cells transfected with STAT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83552 STAT1 antibody [4H9].
Loading : 5 ug per lane

ICC/IF analysis of COS7 cells transiently transfected with STAT1 plasmid using GTX83552 STAT1 antibody [4H9].