SIL1 antibody [3E3], IgG1, Clone: [3.00E+03], Unconjugated, Mouse, Monoclonal

Catalog Number: GTX83642
Article Name: SIL1 antibody [3E3], IgG1, Clone: [3.00E+03], Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: GTX83642
Supplier Catalog Number: GTX83642
Alternative Catalog Number: GTX83642-100
Manufacturer: GeneTex
Host: Mouse
Category: Antikörper
Application: FACS, ICC, IHC-P, WB
Species Reactivity: Human
Immunogen: Full length human recombinant protein of human SIL1 (NP_071909) produced in HEK293T cell.
Conjugation: Unconjugated
Alternative Names: SIL1 nucleotide exchange factor , BAP , MSS , ULG5
Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.
Clonality: Monoclonal
Concentration: 0.56 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: [3.00E+03]
Molecular Weight: 52
Isotype: IgG1
NCBI: 64374
UniProt: Q9H173
Buffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
Source: Human
Purity: Purified by affinity chromatography
Form: Liquid
Application Notes: WB: 1:200. IHC-P: 1:100-500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
IHC-P analysis of liver tissue using GTX83642 SIL1 antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of liver carcinoma tissue using GTX83642 SIL1 antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
FACS analysis of HEK293T cells transfected with either SIL1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83642 SIL1 antibody [3E3].
WB analysis of HEK293T cells transfected with SIL1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83642 SIL1 antibody [3E3].
Loading : 5 ug per lane
IHC-P analysis of kidney tissue using GTX83642 SIL1 antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
ICC/IF analysis of COS7 cells transiently transfected with SIL1 plasmid using GTX83642 SIL1 antibody [3E3].
WB analysis of various cell lines using GTX83642 SIL1 antibody [3E3].
Loading : 10 ug per lane
Dilution : 1:200
GTX83642 ICC/IF Image
IHC-P analysis of human ovary adenocarcinoma tissue using GTX83642 SIL1 antibody [3E3].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50