PIM2 antibody [9A5], IgG2a, Unconjugated, Mouse, Monoclonal

Catalog Number: GTX83892
Article Name: PIM2 antibody [9A5], IgG2a, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: GTX83892
Supplier Catalog Number: GTX83892
Alternative Catalog Number: GTX83892-100
Manufacturer: GeneTex
Host: Mouse
Category: Antikörper
Application: FACS, ICC, IHC-P, IP, WB
Species Reactivity: Human
Immunogen: Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Conjugation: Unconjugated
Alternative Names: Pim-2 proto-oncogene, serine/threonine kinase
Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade | this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via in
Clonality: Monoclonal
Concentration: 0.87 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: [9A5]
Molecular Weight: 34
Isotype: IgG2a
NCBI: 11040
Pubmed: 29985480,
UniProt: Q9P1W9
Buffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
Source: Human
Purity: Purified by affinity chromatography
Form: Liquid
Application Notes: WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX83892 IP Image
WB analysis of HEK293T cells transfected with PIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83892 PIM2 antibody [9A5].
Loading : 5 ug per lane
ICC/IF analysis of COS7 cells transiently transfected with PIM2 plasmid using GTX83892 PIM2 antibody [9A5].
FACS analysis of HEK293T cells transfected with either PIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83892 PIM2 antibody [9A5].
IHC-P analysis of human prostate tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human liver tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human colon tissue using GTX83892 PIM2 antibody [9A5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
FACS analysis of Jurkat cells using GTX83892 PIM2 antibody [9A5].
Red : Primary antibody
Blue : Negative control antibody
FACS analysis of HeLa cells using GTX83892 PIM2 antibody [9A5].
Red : Primary antibody
Blue : Negative control antibody