Janelia Fluor-549-SE-100mug, CAS [[1811539-32-8]]

Product Overview

• Article Name: Janelia Fluor-549-SE-100mug, CAS [[1811539-32-8]]
• Biozol Catalog Number: HLB-HB7336
• Supplier Catalog Number: HB7336
• Alternative Catalog Number: HLB-HB7336-100UG,HLB-HB7336-20UG,HLB-HB7336-2MG,HLB-HB7336-3X100UG,HLB-HB7336-40UG
• Manufacturer: HelloBio
• Category: Biochemikalien
• Alternative Names: JF549, JF549-SE

Product Description

Yellow dye for coupling to NHS groups. Suitable for dSTORM, STED, confocal microscopy, live cell imaging and flow cytometry.

Product Properties

• Molecular Weight: C31H25N3O7
• Pubmed: 124201856
• Source: Synthetic
• Purity: {no attribute purity}
• Form: Red to purple solid
• CAS Number: [1811539-32-8]
• Formula: C31H25N3O7

Application Notes

• Application Notes: Protocol 1: Conjugation of Janelia Fluor 549 SE to antibodies Using a desalting column, perform buffer exchange (following manufacturer instructions) of antibody into a carbonate buffer (100mM, pH 8-8.25) Mix together the antibody and Janelia Fluor 54

Images

β-tubulin expression in HeLa cells visualised using a Janelia Fluor® 549 conjugated secondary antiobody.

GFAP expression in rat brain visualised using a Janelia Fluor® 549 conjugated secondary antibody.

β-tubulin expression in HeLa cells visualised using a Janelia Fluor® 549 conjugated secondary antiobody.

Fig1. Excitation and emission spectra of Janelia Fluor® 549, SE.

HB7336