Protocol 1: Measurement of excitation and emission spectra of Janelia Fluor 549, azide: Janelia Fluor 549, azide was prepared at 1µm in PBS. Spectra were generated on a Tecan Infinite M200 PRO using the following parameters: Excitation: Recording at 6
Fig1. Excitation and emission spectra of Janelia Fluor® 549, azide.
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