Protocol 1: Measurement of excitation and emission spectra of Janelia Fluor 549, free acid: Janelia Fluor 549, free acid was prepared at 1µm in PBS. Spectra were generated on a Tecan Infinite M200 PRO using the following parameters: Excitation: Record
Fig1. Excitation and emission spectra of Janelia Fluor® 549, free acid.
HB8745
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