Mca-YVADAP-K(Dnp)-OH, or Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH, is a highly fluorescent FRET substrate for the determination of caspase-1 (ICE) and caspase-1-like enzyme activities. Cleavage of Mca-YVADAPK(Dnp)-OH at the P1 Asp residue results in a con