Normal Mouse Serum

Catalog Number: JIM-015-000-120
Article Name: Normal Mouse Serum
Biozol Catalog Number: JIM-015-000-120
Supplier Catalog Number: 015-000-120
Alternative Catalog Number: JIM-015-000-120
Manufacturer: Jackson ImmunoResearch
Host: Mouse
Category: Zellen/Zellkultur
Conjugation: Unconjugated

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Application Dilute: For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum.