Biotin-SP-conjugated AffiniPure(TM) Goat Anti-Human IgM, Fc5µ Fragment Specific

Product Overview

• Article Name: Biotin-SP-conjugated AffiniPure(TM) Goat Anti-Human IgM, Fc5µ Fragment Specific
• Biozol Catalog Number: JIM-109-065-043
• Supplier Catalog Number: 109-065-043
• Alternative Catalog Number: JIM-109-065-043
• Manufacturer: Jackson ImmunoResearch
• Host: Goat
• Category: Antikörper
• Species Reactivity: Human
• Conjugation: Biotin-SP (long spacer)

Additional Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgM from other species.

 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Product Properties

• Clonality: Polyclonal
• Isotype: Whole IgG
• Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Form: Whole IgG
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: 1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry