Alexa Fluor 488-conjugated AffiniPure(TM) F(ab)2 Fragment Goat Anti-Human Serum IgA, alpha Chain Specific

Catalog Number: JIM-109-546-011
Article Name: Alexa Fluor 488-conjugated AffiniPure(TM) F(ab)2 Fragment Goat Anti-Human Serum IgA, alpha Chain Specific
Biozol Catalog Number: JIM-109-546-011
Supplier Catalog Number: 109-546-011
Alternative Catalog Number: JIM-109-546-011
Manufacturer: Jackson ImmunoResearch
Host: Goat
Category: Zellen/Zellkultur
Species Reactivity: Human
Conjugation: Alexa Fluor® 488

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the heavy chain of human IgA but not with human IgG or IgM. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with IgA from other species.

 

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Clonality: Polyclonal
Isotype: F(ab')₂ Fragment
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Form: F(ab')₂ Fragment
Antibody Type: Secondary Antibody
Application Dilute: 1:100 - 1:800 for most applications