Alexa Fluor 647-conjugated AffiniPure(TM) F(ab)2 Fragment Goat Anti-Human IgG, Fcgamma Fragment Specific (min X Bov,Hrs,Ms Sr Prot)
Biozol Catalog Number:
JIM-109-606-098
Supplier Catalog Number:
109-606-098
Alternative Catalog Number:
JIM-109-606-098
Manufacturer:
Jackson ImmunoResearch
Host:
Goat
Category:
Antikörper
Species Reactivity:
Human
Conjugation:
Alexa Fluor® 647
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Clonality:
Polyclonal
Isotype:
F(ab')₂ Fragment
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Purity:
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Form:
F(ab')₂ Fragment
Antibody Type:
Secondary Antibody
Application Dilute:
1:100 - 1:800 for most applications
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