Biotin-SP-conjugated AffiniPure(TM) Goat Anti-Rabbit IgG (H+L)

Product Overview

• Article Name: Biotin-SP-conjugated AffiniPure(TM) Goat Anti-Rabbit IgG (H+L)
• Biozol Catalog Number: JIM-111-065-003
• Supplier Catalog Number: 111-065-003
• Alternative Catalog Number: JIM-111-065-003
• Manufacturer: Jackson ImmunoResearch
• Host: Goat
• Category: Antikörper
• Species Reactivity: Rabbit
• Conjugation: Biotin-SP (long spacer)

Additional Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Product Properties

• Clonality: Polyclonal
• Isotype: Whole IgG
• Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Form: Whole IgG
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: 1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry