Biotin-SP-conjugated AffiniPure(TM) Goat Anti-Rabbit IgG (H+L) (min X Hu,Ms,Rat Sr Prot)
Biozol Catalog Number:
JIM-111-065-144
Supplier Catalog Number:
111-065-144
Alternative Catalog Number:
JIM-111-065-144
Manufacturer:
Jackson ImmunoResearch
Host:
Goat
Category:
Antikörper
Species Reactivity:
Rabbit
Conjugation:
Biotin-SP (long spacer)
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse and rat serum proteins, but it may cross-react with immunoglobulins from other species.
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Clonality:
Polyclonal
Isotype:
Whole IgG
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Purity:
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Form:
Whole IgG
Antibody Type:
Secondary Antibody
Application Dilute:
1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry
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