Peroxidase-conjugated AffiniPure(TM) Goat Anti-Rat IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Ms,Rb,Shp Ig)
Biozol Catalog Number:
JIM-112-035-175
Supplier Catalog Number:
112-035-175
Alternative Catalog Number:
JIM-112-035-175
Manufacturer:
Jackson ImmunoResearch
Host:
Goat
Category:
Antikörper
Species Reactivity:
Rat
Conjugation:
Horseradish Peroxidase
Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on rat IgG and with those common to other rat immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Clonality:
Polyclonal
Isotype:
Whole IgG
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Purity:
The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Form:
Whole IgG
Antibody Type:
Secondary Antibody
Application Dilute:
1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates
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