Peroxidase-conjugated AffiniPure(TM) F(ab)2 Fragment Goat Anti-Mouse IgG (H+L)
Biozol Catalog Number:
JIM-115-036-003
Supplier Catalog Number:
115-036-003
Alternative Catalog Number:
JIM-115-036-003
Manufacturer:
Jackson ImmunoResearch
Host:
Goat
Category:
Antikörper
Species Reactivity:
Mouse
Conjugation:
Horseradish Peroxidase
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Clonality:
Polyclonal
Isotype:
F(ab')₂ Fragment
Buffer:
0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Purity:
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Form:
F(ab')₂ Fragment
Antibody Type:
Secondary Antibody
Application Dilute:
1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates
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