Alexa Fluor 488-conjugated AffiniPure(TM) Fab Fragment Goat Anti-Mouse IgG1, Fcgamma Fragment Specific

Product Overview

• Article Name: Alexa Fluor 488-conjugated AffiniPure(TM) Fab Fragment Goat Anti-Mouse IgG1, Fcgamma Fragment Specific
• Biozol Catalog Number: JIM-115-547-185
• Supplier Catalog Number: 115-547-185
• Alternative Catalog Number: JIM-115-547-185
• Manufacturer: Jackson ImmunoResearch
• Host: Goat
• Category: Antikörper
• Species Reactivity: Mouse
• Conjugation: Alexa Fluor® 488

Additional Information

Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG1 but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.

 

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Product Properties

• Clonality: Polyclonal
• Isotype: Fab Fragment
• Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
• Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
• Form: Fab Fragment
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: 1:100 - 1:800 for sequential labeling applications.