AffiniPure(TM) Sheep Anti-Mouse IgG (H+L)

Product Overview

• Article Name: AffiniPure(TM) Sheep Anti-Mouse IgG (H+L)
• Biozol Catalog Number: JIM-515-005-003
• Supplier Catalog Number: 515-005-003
• Alternative Catalog Number: JIM-515-005-003
• Manufacturer: Jackson ImmunoResearch
• Host: Sheep
• Category: Antikörper
• Species Reactivity: Mouse
• Conjugation: Unconjugated

Additional Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Product Properties

• Clonality: Polyclonal
• Isotype: Whole IgG
• Buffer: Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Preservative: None
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Form: Sterile-filtered liquid
• Storage: Storage: Store at 2-8°C under sterile conditions. Prepare working dilution on day of use. Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: 10-20 µg / ml Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.