Peroxidase-conjugated AffiniPure(TM) Donkey Anti-Goat IgG (H+L)

Product Overview

• Article Name: Peroxidase-conjugated AffiniPure(TM) Donkey Anti-Goat IgG (H+L)
• Biozol Catalog Number: JIM-705-035-003
• Supplier Catalog Number: 705-035-003
• Alternative Catalog Number: JIM-705-035-003
• Manufacturer: Jackson ImmunoResearch
• Host: Donkey
• Category: Antikörper
• Species Reactivity: Goat
• Conjugation: HRP

Additional Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule goat IgG. It also reacts with the light chains of other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Product Properties

• Clonality: Polyclonal
• Buffer: Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free). Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish...
• Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
• Form: Freeze-dried solid
• Storage: Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended...
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: 1:500 - 1:5,000 for immunohisto/cytochemistry_x000D_ 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates_x000D_ 1:10,000 - 1:200,000 for Western blotting with ECL substrates_x000D_ Dilution factors are presented in the form...