Peroxidase-conjugated AffiniPure(TM) F(ab)2 Fragment Donkey Anti-Guinea Pig IgG (H+L) (min X Bov,Ck,Gt,Sy Hms,Hrs,Hu,Ms,Rb,Rat,Shp Sr Prot)
Biozol Catalog Number:
JIM-706-036-148
Supplier Catalog Number:
706-036-148
Alternative Catalog Number:
JIM-706-036-148
Manufacturer:
Jackson ImmunoResearch
Host:
Donkey
Category:
Antikörper
Species Reactivity:
Guinea pig
Conjugation:
Horseradish Peroxidase
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule guinea pig IgG. It also reacts with the light chains of other guinea pig immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, syrian hamster, horse, human, mouse, rabbit, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.
F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.
Clonality:
Polyclonal
Isotype:
F(ab')₂ Fragment
Buffer:
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free). Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish...
Purity:
The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Form:
Freeze-dried solid
Storage:
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended...
Antibody Type:
Secondary Antibody
Application Dilute:
1:500 - 1:5,000 for immunohisto/cytochemistry_x000D_ 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates_x000D_ 1:10,000 - 1:200,000 for Western blotting with ECL substrates_x000D_ Dilution factors are presented in the form...
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