Biotin-SP-conjugated AffiniPure(TM) F(ab)2 Fragment Donkey Anti-Human IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Ms,Rb,Rat,Shp Sr Prot)

Product Overview

• Article Name: Biotin-SP-conjugated AffiniPure(TM) F(ab)2 Fragment Donkey Anti-Human IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Ms,Rb,Rat,Shp Sr Prot)
• Biozol Catalog Number: JIM-709-066-149
• Supplier Catalog Number: 709-066-149
• Alternative Catalog Number: JIM-709-066-149
• Manufacturer: Jackson ImmunoResearch
• Host: Donkey
• Category: Antikörper
• Species Reactivity: Human
• Conjugation: Biotin-SP (long spacer)

Additional Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, mouse, rabbit, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

 

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Product Properties

• Clonality: Polyclonal
• Isotype: F(ab')₂ Fragment
• Buffer: Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free). Preservative: 0.05% Sodium Azide
• Purity: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
• Form: Freeze-dried solid
• Storage: Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended...
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: 1:20,000 - 1:400,000 for ELISA and Western blotting using enzyme-conjugated streptavidin 1:500 - 1:5,000 for enzyme immunohisto/cytochemistry 1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry