Cy3-conjugated AffiniPure(TM) Fab Fragment Donkey Anti-Rabbit IgG (H+L)

Product Overview

• Article Name: Cy3-conjugated AffiniPure(TM) Fab Fragment Donkey Anti-Rabbit IgG (H+L)
• Biozol Catalog Number: JIM-711-167-003
• Supplier Catalog Number: 711-167-003
• Alternative Catalog Number: JIM-711-167-003
• Manufacturer: Jackson ImmunoResearch
• Host: Donkey
• Category: Antikörper
• Species Reactivity: Rabbit
• Conjugation: Cyanine Cy™3

Additional Information

Based on antigen-binding assay and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

 

Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

Product Properties

• Clonality: Polyclonal
• Isotype: Fab Fragment
• Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free). Preservative: 0.05% Sodium Azide
• Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
• Form: Freeze-dried solid
• Storage: Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended...
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.