Alexa Fluor 594-conjugated AffiniPure(TM) F(ab)2 Fragment Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)

Product Overview

• Article Name: Alexa Fluor 594-conjugated AffiniPure(TM) F(ab)2 Fragment Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Ms,Rat,Shp Sr Prot)
• Biozol Catalog Number: JIM-711-586-152
• Supplier Catalog Number: 711-586-152
• Alternative Catalog Number: JIM-711-586-152
• Manufacturer: Jackson ImmunoResearch
• Host: Donkey
• Category: Antikörper
• Species Reactivity: Rabbit
• Conjugation: Alexa Fluor® 594

Additional Information

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

 

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Product Properties

• Clonality: Polyclonal
• Isotype: F(ab')₂ Fragment
• Buffer: Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free). Preservative: 0.05% Sodium Azide
• Purity: AffiniPure
• Form: Freeze-dried solid
• Storage: Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended...
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: 1:100 - 1:800 for most applications Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.