Fluorescein (FITC)-conjugated AffiniPure(TM) Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot)

Catalog Number: JIM-715-095-151
Article Name: Fluorescein (FITC)-conjugated AffiniPure(TM) Donkey Anti-Mouse IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu,Rb,Rat,Shp Sr Prot)
Biozol Catalog Number: JIM-715-095-151
Supplier Catalog Number: 715-095-151
Alternative Catalog Number: JIM-715-095-151
Manufacturer: Jackson ImmunoResearch
Host: Donkey
Category: Antikörper
Species Reactivity: Mouse
Conjugation: FITC

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, syrian hamster, horse, human, rabbit, rat and sheep serum proteins, but it may cross-react with immunoglobulins from other species.

 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Clonality: Polyclonal
Isotype: Whole IgG
Buffer: Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free). Preservative: 0.05% Sodium Azide
Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Form: Freeze-dried solid
Storage: Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Extended...
Antibody Type: Secondary Antibody
Application Dilute: 1:50 - 1:200 for most applications