TMB (3, 3', 5, 5'-tetramethylbenzidine) is a chromogenic substrate for Horseradish Peroxidase (HRP). TMB produces a deep blue color during the enzymatic degradation of hydrogen peroxide by HRP, and the addition of a stop solution gives a clear yellow color that absorbs at 450nm.
TMB EASE characteristics are:
1:1 replacement of carcinogenic 2mg OPD
Aqueous based – no organic solvents (REACH Annex XVII compliant)
Low lot-to-lot variation
Form:
Ready-to-use (RTU)
Storage:
2-8⁰C in the dark
Application Notes:
HRP in ELISA – comparable to OPD
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