The secreted recombinant human TLR4 consists of 619 amino acids and predicts a molecular mass of 70.5 kDa. The apparent molecular mass of the protein is approximately 68 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Application Notes:
SDS-PAGE
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