ELISpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-gamma (ALP)

Product Overview

• Article Name: ELISpot Path: SARS-CoV-2 (S1scan+SNMO) Human IFN-gamma (ALP)
• Biozol Catalog Number: MAB-3420-4AST-P1-1
• Supplier Catalog Number: 3420-4AST-P1-1
• Alternative Catalog Number: MAB-3420-4AST-P1-1
• Manufacturer: MABTECH
• Category: Kits/Assays
• Application: ELISpot
• Species Reactivity: Human
• Conjugation: ALP
• Alternative Names: sars-cov-2

Product Description

ELISpot Path kit for enumeration of cells secreting IFN-gamma in response to SARS-CoV-2 peptides (S1scan+SNMO), Each Path kit is specialized for the detection of an antigen-specific response. This unique combination of antigen with immunoassay is validated b

Additional Information

ELISpot Path kit for enumeration of cells secreting IFN-γ in response to SARS-CoV-2 peptides (S1scan+S2N+SNMO)

Each Path kit is specialized for the detection of an antigen-specific response. This unique combination of antigen with immunoassay is validated by scientists at Mabtech.

This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human IFN-γ in response to SARS-CoV-2 peptides.

The kit includes one 96-well ELISpot strip plate (8 wells x 12 strips) pre-coated with monoclonal antibody, SARS-CoV-2 S1 scanning pool, SARS-CoV-2 S2 N defined peptide pool, SARS-CoV-2 SNMO defined peptide pool, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion. Pre-coated plates reduce assay time and minimize variability. In addition, strip-plates are ideal when running a limited number of wells.

The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus. The peptides are 15-mers overlapping with 11 amino acids, covering the S1 domain of the spike protein (amino acid 13-685). The pool is supplied in two vials: pool 1 SARS-CoV-2 S1 peptides 1-83 (83 peptides) and pool 2 SARS-CoV-2 S1 peptides 84-166 (83 peptides).

The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike and nucleoprotein.

The SARS-CoV-2 SNMO defined peptide pool contains 47 synthetic peptides from the human SARS-CoV-2 virus. The peptides are derived from the spike, nucleoprotein, membrane protein, ORF3a and ORF7a.

ELISpot Path: Human IFN-γ (SARS-CoV-2+S1scan+S2N+SNMO) is also available with Streptavidin-HRP and TMB substrate.

---

Analyte Information

IFN-γ
Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

Product Properties

• NCBI: 3458
• Pubmed: 32979941
• Target: IFN-gamma

Application Notes

• Application Notes: Contents: 7-B6-1, biotin,Streptavidin-ALP,BCIP/NBT-plus,Pre-coated ELISpot plate, strip (mAb 1-D1K),Anti-CD3 mAb (CD3-2),Anti-CD28 mAb (CD28-A),SARS-CoV-2 (S1), scanning,SARS-CoV-2 (SNMO), human

Images