100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Source:
This antibody was purified from hybridoma (clone 3E1) supernatant using anti-IgY affinity column. This hybridoma was established by fusion of chicken B cell line MUH1 cell with chicken splenocyte immunized with recombinant Fc fusion SynCAM.
Target:
SynCAM (TSLC1/CADM1)
Application Dilute:
WB: 1 ug/mL (chemiluminescence detection system). IP: 10 µg/200 µL of cell extract from 5x106 cells. ICC: Not tested* *It is reported that 3E1 can be used in this application in the reference number 2), 3) and 6).. IHC: 10 µg/mL (Heat treatment required
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