100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Source:
This antibody was purified from hybridoma (clone 3-19) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3-X63-Ag8 with Balb/c mouse splenocyte immunized with human CENP-A peptide (3-19 aa).
Target:
CENP-A
Application Dilute:
WB: 1 ug/mL. ICC: 10 ug/mL. IHC: 5 µg/mL Heat treatment is necessary for paraffin embedded sections. Microwave oven, 2 times for 10 minutes each in 10 mM citrate buffer (pH 6.5). Other: It is reported that this monoclonal antibody can be used in Chromati
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