Anti-LC3 (Human) mAb, Clone: [4E12], Mouse, Monoclonal

Product Overview

• Article Name: Anti-LC3 (Human) mAb, Clone: [4E12], Mouse, Monoclonal
• Biozol Catalog Number: MBL-M152-3
• Supplier Catalog Number: M152-3
• Alternative Catalog Number: MBL-M152-3
• Manufacturer: MBL
• Host: Mouse
• Category: Antikörper
• Application: FC, ICC, IP, WB
• Species Reactivity: Hamster, Human, Mouse, Rat
• Immunogen: Recombinant human LC3 (MAP1LC3B: 1-120 a.a.)

Product Properties

• Clonality: Monoclonal
• Concentration: 2 mg/mL
• Clone Designation: [4E12]
• NCBI: 81631
• Buffer: 200 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
• Source: This antibody was purified from hybridoma (clone 4E12) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with C3H mouse lymphocyte immunized with the recombinant human LC3 [MAP1LC3B (1-120 aa)].
• Target: LC3

Application Notes

• Application Dilute: WB: 5 µg/mL (chemiluminescence detection system). IP: 5 µg/300 µL of cell extract from 1x107 cells. FCM: 40 µg/mL (final concentration). ICC: 40 µg/mL. Other: Immuno-EM: 20 µg/mL