100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
Source:
This antibody was purified from hybridoma (clone 5D4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Balb/c mouse splenocyte immunized with the recombinant human PRAD1/Cyclin D1.
Target:
Cyclin D1
Application Dilute:
WB: 1 µg/mL (chemiluminescence detection system). IP: 1-10 µg/200 µg of cell extract from 5x106 cells. FCM: 10 µg/mL (final concentration). IHC: 1-10 µg/mL (Heat treatment required for paraffin)
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