Anti-Cyclin D1 (Human) mAb, IgG2a, Clone: [5D4], Mouse, Monoclonal

Product Overview

• Article Name: Anti-Cyclin D1 (Human) mAb, IgG2a, Clone: [5D4], Mouse, Monoclonal
• Biozol Catalog Number: MBL-MD-17-3
• Supplier Catalog Number: MD-17-3
• Alternative Catalog Number: MBL-MD-17-3
• Manufacturer: MBL
• Host: Mouse
• Category: Antikörper
• Application: FC, IHC, IP, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant human PRAD1/cyclinD1

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Clone Designation: [5D4]
• Isotype: IgG2a
• NCBI: 595
• Buffer: 100 µg IgG in 100 µl volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.
• Source: This antibody was purified from hybridoma (clone 5D4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell PAI with Balb/c mouse splenocyte immunized with the recombinant human PRAD1/Cyclin D1.
• Target: Cyclin D1

Application Notes

• Application Dilute: WB: 1 µg/mL (chemiluminescence detection system). IP: 1-10 µg/200 µg of cell extract from 5x106 cells. FCM: 10 µg/mL (final concentration). IHC: 1-10 µg/mL (Heat treatment required for paraffin)