This antibody was purified from hybridoma (clone 5A10) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with recombinant rat PCNA.
Target:
PCNA
Application Dilute:
WB: 5 ug/mL. IP: 5 ug/200 uL of cell extract from 2.5 x 10 6 cells. FCM: 10-50 ug/mL (final concentration). ICC: 10 ug/mL. IHC: 2 ug/mL (Heat treatment required for paraffin)
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