This antibody was concentrated from hybridoma (clone 6F1) supernatant. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with the recombinant human EGF-R cytoplasmic region (648-1186 aa).
Form:
The protein concentration should be 1 mg/mL and the IgG concentration should be 100 µg/mL in PBS 50% glycerol, pH 7.2. No preservatives are contained.
Storage:
-20°C
Target:
EGF
Notes:
For Research Use Only. Not for use in diagnostic procedures.
Application Dilute:
FCM: 1:100 (final concentration), ICC: 1:100, IP: 2-5 µL/250 µL of cell extract from 5x106 cells, WB: 1:1000-1:2000
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