SURE® Expression System for Bacillus subtilis: Controlled gene expression in B. subtilis – a cost-saving alternative to IPTG induced gene expression. IPTG (Isopropyl-ß-D-thiogalactopyranoside) is a widespread, established, and non-degradable inducer. However, since IPTG is a considerably expensive compound, an inexpensive method for induction is desirable. Subtilin is a small peptide antibiotic of 32 amino acids produced by B. subtilis. Subtilin production and regulation is encoded in the chromosome by a cluster of 9 genes, which are transcribed from two promoters. Subtilin regulates/activates its own biosynthesis via a two-component regulatory system. For the SURE system the regulatory components called SpaR (response regulator) and SpaK (membrane sensor) were isolated and placed on the chromosome of a B. subtilis host strain. One of the subtilin regulated promoters is located upstream of a multiple cloning site into which the gene of interest can be cloned. Upon addition of subtilin the system is activated and the protein of interest is produced. It accumulates either intracellularly, or is secreted into the medium. Features: • Tightly controlled gene expression in a bacterium with long history of biotechnology •Genome sequence available • Longstanding genetic engineering experience • Complete set of genetic engineering tools available • Subtilin producing strain cuts costs • Potentially useful for cloning of genes with toxic products • Potential for secretion of gene products • Controlled gene expression for metabolic engineering • All vectors are B. subtilis/E. coli shuttle vectors
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