Mobicol Spin Ni-IDA

Product Overview

• Article Name: Mobicol Spin Ni-IDA
• Biozol Catalog Number: MOB-PR-HTK110
• Supplier Catalog Number: PR-HTK110
• Alternative Catalog Number: MOB-PR-HTK110
• Manufacturer: MoBiTec
• Category: Chromatographie

Additional Information

Ni-IDA Columns for Purification of His-tagged Proteins.

Features:

• Easy, fast, and cost-effective routine purification of recombinant polyhistidine-tagged proteins under native and denaturing conditions starting from diverse expression systems, e.g., E. coli, yeast, insect, and mammalian cells

• Universal use: suitable for small proteins, large protein complexes, proteins with low expression rates

• High binding capacity and high affinity

• Improved target specificity by optimized silica-based Ni2+-IDA matrix

• Imidazol-free loading and washing buffer.

His-Tagged Protein Purification: Histidine-tagged protein purification uses affinity chromatography to capture recombinant proteins with 4 - 10 histidine residues. Ni-IDA columns from MoBiTec contain a silica-based resin. The form-stable silica matrix is pre-charged with Ni2+ ions and allows purification on the principle of Immobilized Metal Ion Affinity Chromatography (IMAC). Binding of proteins is based on the interaction between the polyhistidine tag of a recombinant protein and immobilized Ni2+ ions. The chelating group of the Ni-IDA resin is based on iminodiacetic acid (IDA), which enables strong and efficient binding of target protein to the IMAC matrix. In contrast to traditional IDA matrices, MoBiTec Ni-IDA matrix is an optimized matrix with low density of IDA ligands. This non-saturating surface concentration of IDA eliminates almost all non-specific interactions of contaminating host proteins with the adsorbent. As a result, Lab Columns Ni-IDA and Mobicol Spin Ni-IDA columns provide much higher target protein purity.

Mobicol Spin Ni-IDA Columns:

• Pre-packed, single-use spin columns for simple, small-scale purification of histidine-tagged proteins and rapid expression screening.

• For protein purification by spinning

• Compatible with standard microcentrifuges

• The purification run takes approximately 10 min

• Very high binding capacity: up to 12 mg protein per spin column

• Excellent protein recovery rate of > 90%

• No commercial license required

Images

PR-HTK110