Vector pUC18 and pUC19: The plasmids have a multiple cloning site within the lacZ alpha-fragment. Inserts cloned into this site disrupt beta -galactosidase activity and give rise to white colonies on X-Gal/IPTG plates. The plasmids encode resistance to ampicillin. Foreign DNA inserted in-frame with the lac Z gene will be expressed as a fusion protein (containing a portion of the beta-galactosidase) under control of the lac promoter. The promoter is inducible with IPTG and followed by an initiation codon as well as a ribosome binding site. pUC18 and pUC19 differ in their multiple cloning site orientation. Features: Commonly used cloning vectors that conveys the Amp resistance • Small, high copy cloning vector for replication in E. coli • Suitable for blue-white screening technique • Also available: pUC118 and pUC119 which contain an additional M13 phage origin for single strand production
V33202
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