Vectors for EosFP Photoconvertible Fluorescent Proteins. Photoconvertible Fluorescent Proteins (FPs) are able to change their fluorescence emission spectrum from one maximum to another. This is triggered by exposure to light with a certain wavelength. EosFP was isolated from the stony coral Lobophyllia hemprichii. Initially, the protein matures in a green fluorescent state with an emission maximum at 516 nm. Upon irradiation with violet-blue light the chromophore undergoes an irreversible photoconversion to a red state emitting at 581 nm. The wavelengths required for photoconversion and detection of the green and red fluorescent states can be easily separated. This makes EosFP an excellent choice for localization and co-localization studies and for regional optical marking. MoBiTec offers cloning vectors for constructing protein fusions containing EosFP signal peptide as well as ready-to-use constructs for transfection and subsequent microscopy analysis such as EosFP construct with mitochondrial targeting signal. Features: Diverse EosFP variants • Green to red photoconvertible: UV/blue inducible • Permanent, bright and fast • Photoconvertible and photoactivatable EosFP variant (mIrisFP). Description: We offer many diverse cloning and expression vectors encoding different variants of EosFP, that can be used for several applications like localization studies of cells, cell compartments and fusion proteins in live cells. All our EosFP variants are photoconvertible, applicable to high resolution fluorescence microscopy like PALM and stable at 37 °C and below. Applications: • Localization studies of cells, cell compartments and fusion proteins in live cells or tissues • Counting and cluster analyses • Regional optical marking of cells, cell compartments and EosFP fusion proteins by photoconversion • Pulse chase experiments, measurements of kon- and koff rates, and high-resolution fluorescence microscopy like PALM.
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