Influenza virus is propagated in the MDCK cell line. Virus is purified using sucrose density gradient ultracentrifugation, disrupted in the presence of 0.5% Triton X-100 non-ionic detergent/0.6 M KCl, and heat inactivated.
Concentration:
Protein concentrations generally range from 0.5 to 3.0
Buffer:
0.5% Triton X-100 non-ionic detergent/0.6 M KCl
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