293T Lysate

Catalog Number: NBS-PAT-PK1610-200
Article Name: 293T Lysate
Biozol Catalog Number: NBS-PAT-PK1610-200
Supplier Catalog Number: PAT-PK1610-200
Alternative Catalog Number: NBS-PAT-PK1610-200
Manufacturer: Nordic BioSite
Category: Zellen/Zellkultur
Application: WB
Species Reactivity: Human
293T cells are a human embryonic kidney (HEK) cell line that expresses the large T antigen. The large T antigen is commonly used for transfection assays and is required for transient transfection of plasmids with an SV40 origin of replication. 293T cells
Concentration: 2 mg/ml
Buffer: Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl pH6.8, 0.4% SDS, 5% Glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Application Notes: Heat lysate to 95C for 5 minutes and rapidly cooling before use.