Synthetic peptide (N- and C- terminal peptides were used for immunisation: 1. ASGLGSPSPCSAGSEEEDM & 2. CSRLANRAPEPPPQQVAQQQ) - which peptide the antibody recognises has not been tested.
Alternative Names:
ATP dependent helicase CHD4, Chromodomain helicase DNA binding protein 4, Mi 2 autoantigen 218 kDa protein, Mi 2b, Mi2 beta
CHD4/Mi-2b is a component of the nucleosome remodeling and deacetylase (NuRD) complex which is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex function
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), Western Blotting (0.5-1.0ug/ml),Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (0.5-1ug/ml), ,Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissue
Western Blot Analysis of
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