Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Western Blot (1-2ug/ml), Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Frozen & Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of
Formalin-fixed, paraffin-embedded human Placenta stained with PLAP Monoclonal Antibody (PL8-F6).
Western Blot Analysis of JEG-3 cell lysate using PLAP MAb (PL8-F6).
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