Nucleolin (Marker of Human Cells) Antibody, IgG1, Clone: [364-5 + NCL/902], Mouse, Monoclonal

Product Overview

• Article Name: Nucleolin (Marker of Human Cells) Antibody, IgG1, Clone: [364-5 + NCL/902], Mouse, Monoclonal
• Biozol Catalog Number: NBT-4691-MSM3-P0
• Supplier Catalog Number: 4691-MSM3-P0
• Alternative Catalog Number: NBT-4691-MSM3-P0-20,NBT-4691-MSM3-P0-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Lysate of SU-DHL-1 Nuclei (364-5), Recombinant human NCL protein (NCL/902)
• Alternative Names: NCL, Nucl, Nucleolin, Protein C23

Product Description

Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar ch

Product Properties

• Clonality: Monoclonal
• Clone Designation: [364-5 + NCL/902]
• Molecular Weight: 76kDa
• Isotype: IgG1
• NCBI: 4691
• UniProt: P19338
• Form: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (0.5-1ug/million cells), ,Immunofluorescence (0.5-1ug/ml), Western Blot (0.5-1ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris

Images

Immunofluorescence Analysis of PFA-fixed MCF-