PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody, IgG2a, Clone: [PCNA/694], Mouse, Monoclonal

Product Overview

• Article Name: PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody, IgG2a, Clone: [PCNA/694], Mouse, Monoclonal
• Biozol Catalog Number: NBT-5111-MSM2-P1ABX
• Supplier Catalog Number: 5111-MSM2-P1ABX
• Alternative Catalog Number: NBT-5111-MSM2-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC, WB
• Species Reactivity: Canine, Human
• Immunogen: Recombinant full length human PCNA protein
• Alternative Names: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

Product Description

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1

Product Properties

• Clonality: Monoclonal
• Clone Designation: [PCNA/694]
• Molecular Weight: 36kDa
• Isotype: IgG2a
• NCBI: 5111
• UniProt: P12004
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with

Images

Western Blot Analysis of HepG2 cell lysate using PCNA Monoclonal Antibody (PCNA/694)

Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)

Flow Cytometric Analysis of PFA-fixed HeLa cells using PCNA Monoclonal An