CD45RO (T-Cell Marker) Antibody, IgG2a, Clone: [UCHL-1], Mouse, Monoclonal

Product Overview

• Article Name: CD45RO (T-Cell Marker) Antibody, IgG2a, Clone: [UCHL-1], Mouse, Monoclonal
• Biozol Catalog Number: NBT-5788-MSM1-P1ABX
• Supplier Catalog Number: 5788-MSM1-P1ABX
• Alternative Catalog Number: NBT-5788-MSM1-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: FC, IF, IHC
• Species Reactivity: Human, Monkey, Primate
• Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
• Alternative Names: T200, GP180, LCA, Leukocyte Common Antigen, LY5, Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Product Description

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature a

Product Properties

• Clonality: Monoclonal
• Clone Designation: [UCHL-1]
• Molecular Weight: 180-185kDa
• Isotype: IgG2a
• NCBI: 5788
• UniProt: P08575
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45

Images

Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (UCHL-1).

Flow Cytometry of hPBMC using CD45RO Monoclonal Antibody (UCHL-1).

Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Monoclonal Antibody (UCHL-