Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated wi
200ug/ml of Ab purified by Protein L. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (0.5-1ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-paraffin) (2-4ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, f
Immunofluorescence staining of PFA-fixed HePG2 cells using Tumor Necrosis F
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