p53 Tumor Suppressor Protein Antibody, IgG2a, Clone: [BP53-12], Mouse, Monoclonal

Product Overview

• Article Name: p53 Tumor Suppressor Protein Antibody, IgG2a, Clone: [BP53-12], Mouse, Monoclonal
• Biozol Catalog Number: NBT-7157-MSM1-P1ABX
• Supplier Catalog Number: 7157-MSM1-P1ABX
• Alternative Catalog Number: NBT-7157-MSM1-P1ABX-100
• Manufacturer: NeoBiotechnologies
• Host: Mouse
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Canine, Gallus, Hamster, Human, Monkey
• Immunogen: Recombinant human wild-type p53 protein
• Alternative Names: Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

Product Description

This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in familie

Product Properties

• Clonality: Monoclonal
• Clone Designation: [BP53-12]
• Molecular Weight: 53kDa.
• Isotype: IgG2a
• NCBI: 7157
• UniProt: P04637
• Form: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Antibody Type: Monoclonal Antibody

Application Notes

• Application Notes: Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 mi

Images

Western Blot Analysis of human HeLa cell lysate using p53 Mouse Monoclonal Antibody (BP53-12).

Western blot of p53 (1) A431, (2) MCF7, (3) HEK29